Every chamber layout is listed here, except free rooms and bosses.
Every chamber can be mirrored by the game. The resulting chamber and the spawn points are the same, only mirrored.
Several chambers have 2 possible entrances, right or left. The entrance you take can completely change the spawn points, so both variations are listed when necessary.
For some chambers, the initial spawn point of enemies is always identical (indicated by a red circle).
A secondary spawn point can also happen, usually if the spawned enemies have low value, or you are deep in the biome (indicated by an orange circle).
You can download the raw room layouts here: Hades Chambers Layout.zip (50Mo)
Most of this comes from data gathered by Ellomenop and mods made by him and Paradigm, big thanks to them.